Evaluation of the molecular protocols LAMP Illumigene Malaria and Malachite Green for the detection of asymptomatic infections and their applicability in hemotherapy practices and organ or tissue transplants

Objectives

*Original in Portuguese: Avaliação dos protocolos moleculares LAMP Illumigene Malaria e Malachite Green para detecção de infecções assintomáticas e sua aplicabilidade em práticas de hemoterapia e de transplantes de órgãos ou tecidos

This objective of this project is to evaluate the applicability of the Loop-mediated isothermal amplification (LAMP) Illumigene Malaria LAMP molecular assay, comparing its sensitivity and reproducibility with qPCR, Malachite Green-LAMP and nested PCR techniques, for the detection of asymptomatic Plasmodium infections in samples from blood donors and in organ or tissue donors/recipients for transplants.