Last Updated: 03/07/2024
Studies to demonstrate the feasibility of the use of the malaria LAMP kit for population screening in Peru
Objectives
To evaluate a malaria diagnostic strategy based on the use of highly-sensitive LAMP Pan/Pf kits to detect asymptomatic infections, in the context of the screening campaigns conducted by field-mobile teams (“brigadas”) in the Peruvian Amazon.
Loop-mediated isothermal DNA amplification (LAMP) methodology offers an opportunity for point-of-care (POC) molecular detection of asymptomatic malaria infections. However, there is still little evidence on the feasibility of implementing this technique for population screenings in isolated field settings.
In this study 1167 individuals were recruited from terrestrial (‘road’) and hydric (‘riverine’) communities of the Peruvian Amazon for a cross-sectional survey to detect asymptomatic malaria infections. The technical performance of LAMP was evaluated in a subgroup of 503 samples, using real-time Polymerase Chain Reaction (qPCR) as reference standard. The operational feasibility of introducing LAMP testing in the mobile screening campaigns was assessed based on field-suitability parameters, along with a pilot POC-LAMP assay in a riverine community without laboratory infrastructure.
Jan 2015 — Dec 2016
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