Last Updated: 18/06/2024
Generation of synthetic Plasmodium parasites
Objectives
This research involves creating an artificial malaria parasite using murine malaria parasites that stably maintains genome-scale synthetic chromosomes that were actually synthesized outside cells to provide new advances in artificial cell synthesis in eukaryotes, and are expected to contribute to its promotion.
Plasmodium parasites have extremely simple transcriptional regulation mechanism. In addition, the artificial chromosome technology can be used in the parasites. These biological and technical features may be useful for synthesizing the parasite artificially. To examine this idea, this study attempted to synthesize the rodent malaria parasite, P. berghei. A chromosome cleavage method was developed by the CRISPR / Cas9 method, and a recipient parasite was prepared for chromosome transplantation. Next, a shuttle artificial chromosome was created between Plasmodium parasite and Saccharomyces cerevisiae, and the small chromosome was synthesized using it in S. cerevisiae. The synthesized small chromosome was successfully transplanted into the recipient parasites. These results suggested that the Plasmodium parasite is useful organism for synthesizing the eukaryotic cells.
Jun 2019 — Mar 2022
$57,830
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