Last Updated: 28/11/2025

Efficiency of ACT on malaria treatments and analysis of polymorphisms in drug resistance genes of Plasmodium falciparum

Objectives

*Original title and text were machine translated from Japanese

This project aimed to explore the efficiency of ACT on malaria treatments and analyze the polymorphisms in drug resistance genes of Plasmodium falciparum.

Principal Institution

Nagasaki University, Japan

Principal Investigators / Focal Persons

Haruki Uemura

Partner Institutions

Osaka Metropolitan University (OMU), Japan

Partner Investigators

Rie Isozumi

Rationale and Abstract

No artemisinin-resistant malaria parasites have been reported in Indonesia and it has been confirmed in this research project. Study on efficacy of artemisinin combination therapy (ACT) was conducted with malaria patients in Flores Island and its efficacy was confirmed; no detectable malaria parasites remained in the patient peripheral blood on day 3 of ACT.In addition, no mutation were observed in the Kelch13 propeller region of Plasmodium falciparum isolates from several area of Indonesia. A simple and reliable real-time PCR procedure was then developed for malaria parasite detection and quantitation with this Flores Island field samples. Further analysis of polymorphisms in several drug resistant genes of Plasmodium falciparum revealed origins and spread of two unique K540T and I588F pfdhps genetic variants in Indonesia and alteration of pfcrt genotype polymorphism in an East Kalimantan district after ACT introduction.

Project Outputs

Article: Origins and spread of novel genetic variants of sulfadoxine–pyrimethamine resistance in Plasmodium falciparum isolates in Indonesia

External reference

Project details

Themes

Drug Resistance
Impact of Interventions

Date

Apr 2016 — Mar 2019

Total Project Funding

$160,066

Funding Details

Japan Society for the Promotion of Science (JSPS), Japan