Last Updated: 18/07/2025
Identification, regulation and function of pyrethroid detoxification enzyme gene of Anopheles sinensis
Objectives
Using Anopheles sinensis as a model species, this project aims to:
- identify and classify the P450, CCE and GST superfamilies of genes and conduct research on their evolution through the analysis of the whole genome;
- identify the P450, CCE and GST genes responsible for pyrethroid metabolic detoxification, and investigate their molecular characterization through transcriptome and resequencing analyses;
- elucidate the molecular regulation mechanism of candidate detoxification enzyme genes identified, through genome and resequencing analyses;
- investigate their expression patterns through quantitative PCR in different development stages, different genders and different tissues;
- verify pyrethroid metabolic detoxification function of these candidate genes by RNAi technique.
Mosquito-borne diseases are the main threat to human health, and the control of mosquito-borne diseases mainly depend on mosquito vector control through the use of pyrethroid insecticide. Pyrethroid resistance is considered to be the single biggest obstacle for mosquito vector control; however, the molecular mechanism of pyrethroid resistance is still lack of comprehensive understanding. Anopheles sinensis is a main malaria vector in China and southeastern countries in Asia. In recent years, research suggests that metabolic detoxification be the main mechanism for pyrethroid resistance. Recently, this project has sequenced and annotated the genome of Anopheles sinensis, and also sequenced and preliminarily analyzed the genomes of individuals and transcriptomes of pyrethroid-resistant and -susceptible strains from different geographical populations. This work is of important scientific and application value in understanding pyrethroid resistance mechanism, developing resistance detection molecular techniques, and improving the applied effect of pyrethroid.
Jan 2017 — Dec 2020
$91,250
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