Development of assays to evaluate binding of Plasmodium vivax duffy binding protein with its receptor

Objectives

The overall objective of the project is to develop standardized binding assays to study the interaction of PvRII with DARC for use in the evaluation of binding inhibitory activity of sera from pre-clinical and clinical studies. The specific objectives are as follows:

1. To develop a standardized, quantitative receptor-binding assay to study the interaction of PvRII with DARC and to evaluate the binding inhibitory activity of sera from pre-clinical and clinical studies.
2. To develop a cell-based semi-quantitative receptor-binding assay to study the interaction of PvRII with Duffy positive red blood cells and to evaluate the binding inhibitory activity of sera from pre-clinical and clinical studies.