Study on the Mechanism of the Major Resistance Gene CYP4H14 of Anopheles sinensis in the Resistance of Pyrethroid Insecticides

Anopheles sinensis is one of the most important vectors of diseases in China. Severe insecticide resistance to the most widely used pesticide, deltamethrin, for the purpose of public health and agriculture, in Anopheles sinensis has been found through the vector surveillance. Previous study discovered that P450 enzymes activity enhancement played important role in insecticide resistance in Anopheles sinensis, furthermore, the specific high expression of CYP4H14 was confirmed in resistance-phenotype Anopheles sinensis from different regions by means of transcriptome and microarray expression profiling analysis. In addition, CYP4H14 exhibited the specific binding site to deltamethrin through the space molecular structural model. Previous studies have shown that the deltamethrin resistance in mosquitoes often controlled by a very few “major functional genes”. Accordingly, in this study, the researchers put forward the research hypothesis of “CYP4H14 genes in Anopheles sinensis may play an important role in the occurrence and development of deltamethrin resistance ”. To verify this hypothesis, the in vitro metabolic functional analysis of deltamethrin will be performed by the cloning and confirming of CYP4H14 and its receptor, followed by recombinant protein expression, then, the gene expression inducing of CYP4H14 by exposure to insecticide will be checked in deltamethrin-susceptible population, and the mosquitoes mortality by exposure to deltamethrin checked in deltamethrin-resistant population after the CYP4H14 gene silence, respectively, to further analyze the in vivo functional role. Overall, this study would provide an important scientific basis for insecticide resistance monitoring, and the development of new insecticide formulations.

Project details

Basic Science
Enabling Technologies & Assays
Insecticide Resistance

Jan 2018 — Dec 2019

$36,987

China