Last Updated: 11/12/2025
Molecular mechanism of microRNAs-mediated post-transcriptional regulation of OK blood group antigen/BSG
Objectives
This project aims to analyze whether miR-15b-5p, miR-99b-5p, and miR-501-3p are involved in the post-transcriptional regulation of Basigin (BSG), and their potential target sites and functions in the expression of OK/BSG by cell transfection, dual-luciferase reporter gene assay, qRT-PCR and other experimental methods.
Basigin (BSG) is a multifunctional protein and expressed widely. BSG is up-regulated in tissues/cells in a variety of diseases, and is also the potential target for therapy. Recent studies indicate that BSG expressed on red blood cells, which carries OK blood group antigens, is the receptor of Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5). The PfRh5–BSG interaction is essential for erythrocyte invasion by Plasmodium falciparum. The applicant found that miR-15b-5p, miR-99b-5p, and miR-501-3p might be candidate regulation factors of OK/BSG in the previous research on the relativity between the polymorphisms of OK/BSG and the PfRh5–BSG interaction. The expected results might be helpful for the study of malaria treatment and prevention as well as other physiological and pathological processes which are related to BSG, and also might provide new candidate negative regulators for the therapy of associated diseases targeting BSG. Therefore there might be potential clinical application value.
Jan 2016 — Dec 2018
$26,974
Related Resources
