Last Updated: 02/09/2025
Gene Mapping and Functional Validation of Plasmodium berghei ANKA to Induce Cerebral Plasmodium
Objectives
To perform genetic cross using ANKA and NK65 parasites, cloning and screening recombinant progeny from the cross, genetic linkage analysis of cerebral malaria phenotype of each recombinant progeny, identify genetic locus/loci and candidate genes that correlated with cerebral malaria.
Cerebral malaria is the leading cause of death in patients with severe malaria. The research of cerebral malaria revealed that lymphocyte infiltration of the brain consisting mainly of CD8+ T cells is the main effector mechanism of experimental cerebral malaria. And latest research shows that the effects of CD8+ T in the brain are mainly regulated by parasite, but its components are unclear. Although the Plasmodium berghei genome sequencing has been completed, gene knockout method is difficult to identify the primary components of Plasmodium berghei,which induced cerebral malaria, due to many gaps in genome. Genetic hybrid technology is a powerful tool for identification of Plasmodium-related phenotype, and our previous work confirmed that P.b ANKA infection can induce experimental cerebral malaria occurs, but P.b NK65 will not.
Then using gene knockout to evaluate the function of candidate genes.This is a new perspective of understanding the immunopathogenesis of cerebral malaria, and may provide new targets and ideas for the prevention and treatment of cerebral malaria.
*The original title has been translated to English
Jan 2014 — Dec 2016
$36,000
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