Last Updated: 15/11/2024
Molecular characteristics of hemoglobin disease under malaria selection pressure in China-Myanmar border and malaria resistance
Objectives
The study aim is to get hemoglobinopathy prevalence by investigating the hemoglobinopathy incidence in the healthy populations and malaria patients at China-Myanmar border malaria epidemic areas.
The specific objectives of this study are to:
- reveal the hemoglobinopathy molecular characteristics and gene mutation spectrum using the methods of PCR reverse dot hybridization, Gap-PCR, multiple amplify refractory mutation system (MARMS) and PCR-sequencing;
- analyze malaria parasite density using microscopy, genotyping parasite antigen genes of PfMSP1, PfMSP2, PfGLURP and PvMSP3α by nested PCR method; and
- to determine the hemoglobinopathy resistance to malaria and plasmodium population genetic characteristics.
Hemoglobinopathy belongs to autosomal recessive genetic disorder, which divided into thalassemia and abnormal hemoglobin disease, and happens worldwide including the Mediterranean region, Africa, southeast Asia and south of China. Currently it is generally accepted that hemoglobinopathy incidence related to malaria selection pressure, but the mechanism keeps unclear. The study will determine the hemoglobinopathy incidence rate and elucidate the interaction between two disease under malaria selection stress in the region.
Jan 2018 — Dec 2021
$56,591
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