Last Updated: 03/09/2024
Biophysical characterization of the golgi structuring and compacting protein (GRASP) of Plasmodium falciparum
Objectives
*Original in Portuguese: Caracterização biofísica da Proteína de Estruturação e Compactação do Golgi (GRASP) de Plasmodium falciparum
In this project, the objective is to investigate the biophysical/biochemical properties of GRASP from P. falciparum (PfGRASP), the causative agent of malaria. Specifically, the aim is to express, purify and characterize the behavior of PfGRASP in solution, comparing it with the intrinsic disorder pattern previously observed by our group in other GRASPs. Additionally, the liquid-liquid phase separation capability of PfGRASP will be tested, thus helping to determine whether the formation of protein biocondensates is another property common to all members of the family.
GRASPs, a family of peripheral membrane proteins, were initially recognized as essential factors in the reconstitution of Golgi cisternae after mitotic events. More recently, their involvement in unconventional protein secretion (UPS) pathways has emerged as a more significant aspect than their role as Golgi structural factors. The UPS has received considerable attention due to its crucial role in cellular homeostasis and the implications of its dysregulation. However, many of the molecular mechanisms underlying UPS are still poorly understood. Several central features have been identified in UPS pathways, particularly their use in responses to cellular stress. The precise role of GRASPs in these UPS pathways is only beginning to be revealed.
Sep 2023 — Aug 2024
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