Last Updated: 31/01/2025
Tracking back target-site insecticide resistance mechanisms in An. gambiae s.l. populations from Senegal
Objectives
The purpose of this proposals is to retrospectively assess the nationwide situation of insecticide resistance mechanism in wild populations of An. gambiae s.l.
We will estimate:
- Relative proportion of the different species belonging to the An. gambiae complex in each of the selected sentinel sites;
- Allelic frequencies of the main insecticide resistance mechanisms;
- Haplotype frequencies for each molecular marker to infer the origin and evolutionary history of each resistance allele found.
The global malaria response is currently facing many challenges, including the emergence of parasite resistance to antimalarial medicines and mosquito resistance to insecticides. In this situation where the progress against malaria has stalled in many countries, necessitating global plan for insecticide resistance management. A key action of such a plan is the filling the current gaps about the main insecticide resistance mechanisms.
The purpose of this project is to retrospectively track the resistance mechanisms emergence and spread in Anopheles gambiae s.l. natural populations in Senegal.
A random stratified sample will be drawn from an existing collection of An. gambiae s.l. to capture as much as possible the heterogeneity across Senegal. Then the species composition within Anopheles gambiae complex will be determined in each sentinel sites of Senegalese NMCP. Finally, all the putative resistance mechanisms will be screened to infer their origin and evolutionary history. The outcome of this project will inform the Senegalese NMCP to make decisions on what insecticide could be used for better control of resistant malaria vector populations.
Moreover, this project will serve a base to reinforce the collaboration between the “Laboratoire d’Ecologie Vectorielle et Parasitare” (LEVP) of UCAD and the Vector Group at the Medical Research Council Unit-LSTMH of The Gambia, where the advanced laboratory works will be done using the sequencing and genomic platform.
Retrospective study of insecticide resistance mechanism in wild senegalese populations of An. gambiae s.l.
- Samples selection from an existing collection of An. gambiae s.l. from the LEVP biobank collected since 2007.
- Genotyping of main molecular markers of insecticide resistance (Vgsc-1014F/S, Gste2-114T, ace1-119S) using a high throughput multi-locus insecticide resistance marker panel 12 to track resistance emergence and evolution.
- Further characterization of resistance emergence and evolution using the Sanger partial sequencing to infer the haplotype diversity and phylogenetic historic of putative resistance mechanisms.
Oct 2019 — Jun 2022
$181,919
Related Resources
