Last Updated: 19/02/2025

Development of a highly-sensitive HRP2 ELISA

Objectives

To develop a new laboratory test to detect Plasmodium falciparum malaria infection in asymptomatic individuals.

Principal Institution

Partner Institutions

Rationale and Abstract

The aim of this project is to develop a new enzyme-linked immunosorbent assay (ELISA) for the qualitative detection of the presence of histidine-rich protein 2 (HRP2) of Plasmodium falciparum malaria. The Alere™ Malaria Ag P.f HRP2 ELISA has a limit of detection of 25pg/ml for recombinant HRP2 using a 50ul sample volume of whole blood. This new ELISA kit may be used as a laboratory confirmation test for reference panel validation with standard positive samples and could be used as a reference method for highly sensitive HRP2-based tests for malaria such as the ultra-sensitive Alere™ Malaria Ag Pf.

Clinical evaluation and technical support are provided by PATH and FIND.

Project Outputs

Article: Performance of a High-Sensitivity Rapid Diagnostic Test for Plasmodium falciparum Malaria in Asymptomatic Individuals from Uganda and Myanmar and Naive Human Challenge Infections

External reference

BMGF project details

Themes

Asymptomatic Reservoir
Diagnostics
Enabling Technologies & Assays

Date

Jun 2015 — Dec 2017

Total Project Funding

$545,143

Funding Details