Last Updated: 01/03/2024

Molecular epidemiological research and new genome decipherment of Lemur malaria parasite

Objectives

*The details were machine translated from Japanese
The purpose of this research is to conduct molecular epidemiology and novel genome analysis of malaria parasites parasitic on wild lemurs in Madagascar.

Principal Investigators / Focal Persons

Hajime Honma

Rationale and Abstract

Comprehensive detection of pathogens by amplicon metagenomic analysis was performed on fecal specimens of lemurs and their relatives provided by zoos in Japan. First, individual identification analysis was performed by microsatellite analysis on samples collected non-invasively. Faecal DNA from specimens identified as shed from a separate individual was then used to obtain amplicons targeting the prokaryotic 16S rRNA gene and the eukaryotic 18S rRNA gene by PCR, followed by the Illumina MiSeq. Sequence analysis was performed in A bioinformatics analysis pipeline was constructed to clarify the community structure of microorganisms and parasites in feces using the obtained sequence data. Using the constructed pipeline, we are proceeding with the analysis of sequence data, and are beginning to clarify the diverse biotic communities that are composed of fecal specimens. In the results obtained so far, various species such as protozoa such as Blastocystis, amoeba, and trichomonas, and nematodes such as strongyloidia have been detected in lemur feces. Visualization of eukaryotic and prokaryotic communities in faeces by non-metric multidimensional scaling (NMDS) revealed that faecal communities differed depending on the host monkey species. was suggested.

Date

Apr 2019 — Mar 2023

Total Project Funding

$37,351

Funding Details
Japan Society for the Promotion of Science (JSPS)

Grant ID: 19K07527
4,290,000 JPY
Project Site

Japan

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